cg makeprimers regionfile maxsize prefsize dbdir ?minfreq? ?threads?
Make sequencing primers for Sanger validation experiments
This command makes primers for sequencing target regions of the genome. It will try to avoid having known SNPs (from dbSNP) or repeats in the primers. If these canot be avoided, it will annotate the primers with this info. Variants close together can be grouped into regions first using the command:
cg makeregions selvariantlist.tsv 200 > regionfile.tsv